SibEnzyme Ltd (Russia) is the only supplier of the novel type of DNA endonucleases: 5mC-directed site-specific DNA endonucleases.
In epigenetics a research work usually is connected with a study of DNA methylation in region of interest. The gold standard for DNA methylation analysis is sequencing of bisulphite converted DNA. However method of bisulphite conversion is quite sophisticated and often results in obtaining false positive data.
Alternative to bisulphite conversion is the enzymatic method to determine DNA methylation, which is based on the restriction enzymes sensitivity to DNA methylation. For example, HpaII-PCR assay, based on HpaII (recognition site CCGG) that cleaves DNA sequence CCGG, but doesn't cut C(5mC)GG. 
A principal difference of new type of enzymes, introduced by SibEnzyme, is that they cleave only methylated DNA. Besides they have 9 different recognition sites, one of which, GlaI recognition site, is   completely equal to the modification site of DNMT3, which is responsible for DNA methylation de novo.
On the basis of GlaI SibEnzyme recently developed an easy and reliable method for detection of R(5mC)GY site in a desired position of genomic DNA (GLAD-PCR assay).
This method can be used for epigenetic diagnostics, for example, for early cancer detection.
MD DNA endonucleases are a good instrument to study human and mammalian DNA methylation.

Available 9 different recognition sites:


recognition sites


Related links:

  1. Substrate specificity of new methyl-directed DNA endonuclease GlaI
  2. Epigenetic typing of human cancer cell lines by BlsI- and GlaI-PCR assays
  3. BlsI- and GlaI-PCR assays – a new method of DNA methylation study
  4. Available MD-DNA Endonucleases
  5. Poster “GLAD PCR analysis of aberrant DNA methylation in cancer”
  6. Patent “Method for determining nucleotide sequence R(5mC)GY in given position of continuous DNA

Additional info (PDF-files):

  1. MD DNA Endonucleases
  2. GLAD-PCR assay
  3. Early cancer detection
  4. Sibenzyme enzymes list